Analysis Note

ControlPBMCs

Application

Research Sub CategoryImmunoglobulins & Immunology

Research CategoryInflammation & Immunology

Detect CD43 using this Milli-Mark Anti-CD43-FITC Antibody, clone DF-T1 validated for use in FC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

CD43 is a human heavily 0-glycosylated transmembrane protein, which is expressed by T cells and myeloid lineage cells (Borche, 1987; Stross, 1989). Reactivity with plasma cells has been observed (Beschomer, 1999). The molecule is known to be defective in the Wiskott-Aldrich syndrome and may participate in T-cell activation (Stross, 1989).
Immunoblotting studies have revealed molecular mass heterogeneity displayed by the antigen extracted from white blood cells of different lineage. Thus, two major bands of 140 and 130 kDa are seen for the promyelocytic cell line HL60, 110 kDa for the myeloblast cell line KG1 and a major 115 kDa band for peripheral blood lymphocytes (Stross, 1989).

Immunogen

Myeloblast cell line KG1

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Purified Mouse monoclonal IgG1 conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.

Quality

Evaluated by flow cytometry using human PBMCs

Specificity

Antibody recognizes Human CD43 antigen.

Storage and Stability

Maintained refrigerated at 2-8°C from date of receipt. Protected from light in undiluted aliquots for 6 months from date of receipt.

Target description

110 kDa (Stross, 1989)