Milli-Mark Anti-TrkA-FITC

Analysis Note

ControlHeLa cells

Application

Research Sub CategoryNeurotransmitters & ReceptorsNeurodegenerative Diseases

Research CategoryNeuroscience

Detect TrkA using this Milli-Mark Anti-TrkA-FITC Antibody validated for use in FC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

TrkA is a high affinity nerve growth factor (NGF). The Trk proto-oncogene family contains four members, TrkA, TrkB, TrkC, and TrkE, which are variably expressed throughout the central and peripheral nervous systems. TrkA binds to nerve growth factor (NGF) and autophosphorylates on tyrosine residues (Tyr490, Tyr674, Tyr675, Tyr751 and Tyr785) to activate multiple downstream effector proteins. Phosphorylation at Tyr490 is required for Shc association and subsequent activation of the Ras-MAP kinase-signaling cascade, which leads to activation of Elk-1-dependent gene transcription and neurite growth. Phosphorylations at Tyr674 and Tyr675 lie within the catalytic domain of TrkA tyrosine kinase and reflect Trk kinase activity. Additionally, phosphorylation at Tyr751 is required for PI3-kinase association and activation of the Akt signaling cascade.

Immunogen

Epitope: Extracellular domain

Bacterially expressed purified protein corresponding to the entire extracellular domain of rat TrkA receptor.

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Protein A purified

Purified rabbit polyclonal IgG conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Quality

Evaluated by flow cytometry using HeLa cells.

Specificity

Antibody recognizes TrkA protein.

Unconjugated version Cat.No.06-574 is predicted to react with Rat based on immunogen design. The unconjugated part number was also shown to react with Mouse.

Storage and Stability

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Target description

TrkA exists as two isoforms: 87 kDa and 88 kDa