Analysis Note

Positive ControlSV80 or SV-T2 cells

Application

Immunoprecipitation (15 µl suspension/sample)r>Affinity Purification (see comments)

General description

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Supplied as an agarose conjugate. Recognizes the ~94 kDa SV40 large T antigen.

This Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416) Agarose Conjugate is validated for use in Immunoprecipitation, Affinity Purification for the detection of SV40 T Antigen (Ab-2).

Recognizes the ~94 kDa SV40 large T antigen in SV80 and SV-T2 cells. Cat. No. DP02A is linked to agarose for immunoprecipitation and purification.

Immunogen

Epitope: Within amino acids 83-128

purified SV40 large T-antigen

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Suitable for affinity purification and immunoprecipitation. The amount of suspension required for purification is dependent on individual sample types.

DeCaprio, J.A., et al. 1988. Cell54, 275.Whyte, P., et al. 1988. Nature334, 124.Mitchell, P.J., et al. 1987. Cell50, 847.Dixon, R.A.F. and Nathans, D., 1985. J. Virol.53, 1001.Simanis, V. and Lane, D.P., 1985. Virol.144, 88. Mann, R.S. and Carroll, R.B. 1984. Virology138, 379 Sarnow, P., et al. 1982. Cell28, 387.Crawford, L.V., et al. 1981. Proc. Natl. Acad. Sci. USA78, 41.Lane, D.P. and Crawford, L.V. 1979. Nature278, 261.Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA71, 3754.Tooze, J.1973. Cold Spring Harbor, New York.Tegtmeyer, P. 1972. J. Virol.10,

Packaging

0.5 ml in Glass bottle

Please refer to vial label for lot-specific concentration.

Physical form

50% slurry in PBS.

Warning

Toxicity: Standard Handling (A)