Anti-Fibronectin (Ab-3) Mouse mAb (FBN11)

Analysis Note

Positive ControlSW 156 cells or normal kidney tissue

Application

Frozen Sections (0.5-1 µg/ml) r>Immunoblotting (0.5-1 µg/ml) r>Immunofluorescence (see comments)r>Immunoprecipitation (2 µg/mg protein lysate) r>Paraffin Sections (0.5-1 µg/ml, heat pre-treatment required)

General description

Recognizes the ~440 kDa disulfide-linked, dimeric fibronectin in SWI56 cells and normal kidney tissue.

Protein G purified mouse monoclonal antibody. Recognizes the ~440 kDa disulfide-linked, dimeric fibronectin.

Anti-Fibronectin (Ab-3), mouse monoclonal, clone FBN11, recognizes dimeric fibronectin in SWI56 cells and normal kidney. It is validated for use in WB, IF, IP & IHC (frozen and paraffin sections).

Immunogen

recombinant human fibronectin

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Clark, R.A. 2001. Ann. N.Y. Acad. Sci.936, 355.Armstrong, P.B. and Armstrong, M.T. 2000. Biochim. Biophys. Acta1470, O9.Barilla, M.L. and Carsons, S.E. 2000. Semin. Arthritis Rheum.29, 252.Yamada, K.M. 1989. In Fibronectin, Mosher, D.F., ed., p. 47.Rouslahti, E. and Pierschbacher, M.D. 1986. Cell44, 517.Pytela, R., et al. 1985. Cell40, 191.

Exhibits staining of the connective tissue matrix when used in immunohistochemistry. This antibody is suitable for both reduced and non-reduced protein by immunoblotting. For staining of formalin-fixed tissues, boil in 10 mM citrate buffer, pH 6.0, for 10-20 min, followed by cooling at room temperature for 20 min. This antibody has also been reported to work for immunofluorescence. Antibody should be titrated for optimal results in individual systems.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)