UG ANTI-A-TUBULIN MOUSE MAB (DM1A)

Analysis Note

Negative Controltrp E (Ab-1)

Positive ControlAny cell line

Application

Immunoblotting (20-100 ng/ml, colorimetric or chemiluminescence; see application references)r>Immunofluorescence (5 µg/ml)

General description

This Anti-α-Tubulin Mouse mAb (DM1A) is validated for use in Immunoblotting, Immunofluorescence for the detection of α-Tubulin.

Recognizes the ~60 kDa tubulin protein found in all eukaryotic cells. May be used as a positive control.

Purified mouse monoclonal antibody generated by immunizing Balb/c57 mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~60 kDa tubulin protein.

Immunogen

native chick brain microtubules

Chicken

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Blose, S.H., et al. 1984. J. Cell. Biol.98, 847.Lin, J.J.C., et al. 1984. In: Monoclonal antibodies and functional cell lines. Kennett, R.H., Bechtol, K.B. and McKearn, T.J., eds. Plenum Publishing Corp.Lin, J.J.C. 1982. Cold Spring Harbor Symp. on Quant. Biol.46, 769.Goldman, R.D., et al. 1979. Ann. Rev. Physiol.41, 701.Goldman, R., Pollard, T. and Rosenbaum, J. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C, Cold Spring Harbor, New York.

Tubulin is found in all cells and is used as a positive control for immunofluorescence or immunoblotting. Antibody should be titrated for optimal results in individual systems.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin.

Warning

Toxicity: Standard Handling (A)