Analysis Note

Positive ControlJunkat or LS174T cells

Negative ControlNormal mouse serum

Application

Immunoblotting (1 g/ml)Immunoprecipitation (2 g/mg protein lysate)

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the ~55 kDA Cdh1 protein.

Anti-Cdh1 (Ab-2), mouse monoclonal, clone DH01, recognizes the ~55 kDa Cdh-1 protein in Rat-1 cells. It is validated for Western blotting and immunoprecipitation.

Recognizes the ~55 kDa Cdh-1 protein in Rat-1 cells.

Immunogen

recombinant human Cdh1 protein

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Does not cross-react with other WD repeat containing proteins, including Cdc20. Antibody should be titrated for optimal results in individual systems.

Kramer, E.R., et al. 2000 Mol. Biol. Cell11, 1555.Petersen, B.O., et al. 2000 Genes Dev.14, 2330.Pfleger, C.M., et al. 2001 Genes Dev.15, 1759.Sorensen, C.S., et al. 2001 Mol. Cell Biol.21, 3692.Gieffers, C., et al. 1999 Proc. Natl. Acad. Sci. USA96, 11317.Visintin, R., et al. 1997 Science278, 460.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 10 mM PBS, 0.2% BSA, pH 7.4.

Warning

Toxicity: Standard Handling (A)