Application

This Anti-PECAM-1 Antibody, clone 390, FITC conjugated is validated for use in FC, IH for the detection of PECAM-1.

Flow Cytometry: = 1 µg per 10e6 cells in 100 µL volume

Immunohistochemistry: acteone fixed frozen tissue sections.

Optimal working dilutions must be determined by the end user.

Research CategoryCell Structure

Research Sub CategoryAdhesion (CAMs)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

PECAM-1 is a type I integral membrane glycoprotein, and a member of the immunoglobulin superfamily of cell surface receptors. PECAM-1 is constitutively expressed on the surface of endothelial cells where it is concentrated at cell junctions. PECAM-1 is also expressed at low levels on all leukocytes and platelets. Homotypic interaction of CD31 is important in cell-cell interaction, cell-matrix interaction and signal transduction.

Immunogen

Mouse leukocyte cell line 32D

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified immunoglobulin conjugated to fluorescein. Liquid in in phosphate buffered saline containing 0.1% sodium azide.

Specificity

CBL1337 recognizes mouse PECAM-1, a 130-140 kDa type 1 integral membrane glycoprotein. This antibody has been shown to inhibit the aggregation of L cells transfected with a variant form of PECAM-1 (DeLisser, 1993).

Storage and Stability

Maintain at 2°-8°C for up to 12 months from date of receipt. DO NOT FREEZE.