Anti-ACTN4 Mouse mAb (4D10)

Analysis Note

Negative Control293T cells

Positive ControlPC-12 cells, HeLa cells, Raw 264.7 cells, MCF-7 cells, NIH3T3 cells, Human testes tissue

Application

ELISA (see coments)Immunoblotting (1-5 g/ml)Immunocytochemistry (20 g/ml)Paraffin Sections (3 g/ml)

General description

Anti-ACTN4, mouse monoclonal, clone 4D10, recognizes the ~90-100 kDa ACTN4 in PC-12, HeLa, Raw 264.7, MCF-7, NIH3T3 cells & in human testes. It is validated for ELISA, WB, ICC, and paraffin sections.

Purified mouse monoclonal antibody. Recognizes the ~90-100 kDa ACTN4 protein.

Recognizes the ~90-100 kDa ACTN4 protein in PC-12, HeLa, Raw 264.7, MCF-7, and NIH3T3 cells and human testes tissue.

Immunogen

A recombinant polypeptide corresponding to amino acids 592-702 of human ACTN4, espressed as a GST fusion protein

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Yamada, S., et al. 2010. Int. J. Oral Maxillofac. Surg.39, 61.

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Regulatory Review (Z)