Anti-GAPDH Mouse mAb (6C5)

Analysis Note

Positive ControlRat skeletal muscle

Application

General description

Protein A purified mouse monoclonal antibody. Recognizes the ~146 kDa GAPDH protein under non-reducing conditions and the ~36 kDa monomeric subunits under reducing conditions.

Recognizes the ~146 kDa GAPDH protein under non-reducing conditions and the ~36 kDa monomeric subunits under reducing conditions.

Anti-GAPDH, mouse monoclonal, clone 6C5, recognizes ~146 kDa GAPDH protein under non-reducing conditions & 36 kDa monomers under reducing conditions. It is validated for use in ELISA, WB, and IHC.

Immunogen

Rabbit

purified, rabbit muscle

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Useful as a loading or positive control since GAPDH is expressed in all cells. Also reported to work for ELISA and immunocytochemistry. Antibody should be titrated for optimal results in individual systems.

Packaging

Please refer to vial label for lot-specific concentration.

500 µg in Plastic ampoule

Physical form

In PBS, pH 7.4.

Reconstitution

Following initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.

Warning

Toxicity: Highly Toxic (H)