Analysis Note

The antibody was characterized on ceramide covalently bound to myoglobin.

Application

Monoclonal Anti-Ceramide antibody produced in mouse has been used in:immunohistochemistryimmunolabeling in electron microscopyenzyme-linked immunosorbent assay (ELISA)immunoblottingimmunoprecipitationas a probe to determine the presence and roles of ceramide in sphingomyelin pathway signaling and the regulation of protein phosphorylation.

Biochem/physiol Actions

Ceramide appears to have a role in mediating biological responses in a wide variety of cell types. Ceramide metabolites such as sphingosine and sphingosine-1-phosphate have potent biological activities of their own. They are directly involved in the proliferation and differentiation of skin cells and regulate skin barrier functionality. Ceramide based analogs are potential anti-tumor agents and are regarded as tumor suppressor lipid. Mechanisms for ceramide action involve regulation of protein phosphorylation via stimulation of a serine/threonine protein phosphatase, a proline-directed kinase and possibly other direct and/or indirect targets. Ceramide is emerging as an intracellular messenger than mediates effects on terminal differentiation and cell proliferation as well as apoptosis or cell death and cell-cycle arrest. The interrelationship of ceramide actions with other bioactive lipids and systems represents an area of active research.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Ceramide is an endogenous lipid component of a novel biochemical pathway termed the sphingomyelin pathway. Ceramide is produced in response to cellular stimulation by hormones, cytokines and antigens. Ceramide synthesis is mediated either by the salvage pathway by the acylation of sphingosine or sphingolipids hydrolysis or by de novo pathway via dihydroceramide formation. Structurally, ceramides have a sphingoid base, a long-chain amino alcohol linked to fatty acid by an amide bond. Ceramide is generated by hydrolysis of sphingomyelin by sphingomyelinase.

Immunogen

ceramide conjugated to bovine serum albumin.

Physical form

Solution in phosphate buffered saline containing 0.5 M NaCl, 0.1% bovine serum albumin, and 0.09% sodium azide.

Preparation Note

Purified from ascites fluid by gel filtration using sephacryl S-300 resin.

Specificity

Mouse monoclonal clone MID 15B4 anti-Ceramide antibody recognizes free and bound ceramides.