Application

Anti-Cy5 antibody, Mouse monoclonal has been used in immunofluorescence assays and western blotting.

Mouse monoclonal clone CY5-15 anti-Cy5 antibody is used to tag CyDye 5 for detection and quantitation and signal applification by or within immunocytochemical and immunohistochemical (IHC) techniques such as ELISA, immunoprecipitation, immunocytochemistry, dot blot assay, and protein microarrays. Anti-Cy5 antibody is an important tool for Cy5 signal amplification in assays where biomolecules are labeled with Cy5.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Mouse monoclonal clone CY5-15 anti-Cy5 antibody recognizes Cy5 and AlexaFluor647 labeled proteins, but not Cy3 labeled proteins.

CyDyes are a family of fluorophores that can be used for labeling proteins, peptides, DNA, RNA, and other biomolecules. These dyes are small, pH insensitive (between pH 3 and pH 10), and soluble in aqueous solution and have tolerance to DMSO. They are more photo-stable than fluorescein, have high molar extinction coefficients, and favorable quantum yields. Due to their physical and chemical properties, they are used (mainly Cy3 and Cy5) in many different biological assays such as: DNA microarrays, protein microarrays, two-dimensional protein analysis (2D gels), fluorescence resonance energy transfer (FRET), and immunocytochemistry.

Immunogen

mixture of proteins labeled with Cy5.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

The antibody recognizes Cy5 and AlexaFluor 647 labeled proteins, but not Cy3 labeled proteins.

Monoclonal Anti-Cy5 recognizes Cy5 and AlexaFluor647 labeled proteins, but not Cy3 labeled proteins.