Application

Anti-RNF146 polyclonal antibody is used to tag ring finger protein 146 for detection and quantitation by Western blotting and in plasma by immunohistochemical (IHC) techniques. It is used as a probe to determine the roles ring finger protein 146 in Wnt signaling and neuroprotection.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Ring finger protein 146 (RNF146, Iduna) is a poly(ADP-ribose) polymer binding and PARsylation dependent E3 ubiquitin ligase which ubiquitinates proteins for degradation by proteosomes. RNF146 is neuroprotective due to its inhibition of Parthanatos via binding with Poly(ADP-ribose) (PAR). RNF146 regulates Wnt signaling via ubiquitylation of auto-PARsylated tankyrase.

Immunogen

Synthetic peptide directed towards the C terminal region of human RNF146

Physical form

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Sequence

Synthetic peptide located within the following region: AVVAQHSLTQQRLLVSNANQTVPDRSDRSGTDRSVAGGGTVSVSVRSRRP

Specificity

Anti-RNF146 polyclonal antibody reacts with human and mouse ring finger protein 146 proteins.