Application

Goat anti-Human IgM+IgG+IgA Antibody, F(ab′)2, biotin conjugate is an antibody against Human IgM+IgG+IgA for use in ELISA & IF.

Research Sub CategoryFragment Specific Secondary Antibodies

Research CategorySecondary & Control Antibodies

Immunofluorescence: ≤1 µg/10⁶ cells.
ELISA: 1:5,000-1:20,000.

Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

IgG is the most abundant immunoglobulin comprising two heavy chains and two light chains. Each molecule has two antigen binding sites. IgG has 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). IgM constitutes about 10% of serum immunoglobulins. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces.

Immunogen

Epitope: Human IgM, IgG, IgA

Prepared from pepsin digest of goat anti-human Ig.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified by gel filtration chromatography of pepsin digested antibody. Liquid in 1.0 mL PBS/NaN₃.

Gel filtration chromatography

Specificity

The antibody reacts with the heavy and light chains of human IgM, IgG and IgA, and with the light chains of human IgD as demonstrated by ELISA and flow cytometry.

Reacts with the heavy and light chains of human IgM, IgG and IgA, and with the light chains of human IgD.

Storage and Stability

Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt.