Anti-Human Ig; lambda;Gt; AP 1ML

Application

Research Sub CategoryFragment Specific Secondary Antibodies

Research CategorySecondary & Control Antibodies

This Goat anti-Human Ig λ chain Antibody, Alkaline Phosphatase conjugate is validated for use in ELISA for the detection of Human Ig λ chain.

ELISA: 1:2,000-1:4,000.

Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Antibody molecules typically comprise two immunoglobulin light chains covalently bound to a pair of heavy chains. Immunoglobulin light chains occur in two types, designated by the Greek letters kappa and lambda. Kappa and lambda light chains are approximately 250 amino acids in length with an average mass of about 25 kDa. The ratio of kappa to lambda found in the immunoglobulin population varies by species.

Immunogen

Epitope: Ig lambda chain

Prepared from pooled antisera from goats hyperimmunized with human myeloma proteins with lambda light chains.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified by affinity chromatography on pooled human Igs with lambda light chains covalently linked to agarose. Liquid in 50mM Tris/1mM MgCl₂/50% Glycerol, pH 8.0, containing 0.1% NaN₃ as preservative.

Affinity Chromatography

Specificity

The antibody reacts with human lambda light chains as demonstrated by ELISA and flow cytometry.

Reacts with human lambda light chains. Absorbed for human myeloma proteins with kappa light chains.

Storage and Stability

Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. For long term storage, aliquote and store at -20°C. Avoid repeated freeze/thaw cycles.