Application

Research Sub CategorySecondary Antibodies Adsorbed for Dual LabelingFragment Specific Secondary Antibodies

Detect Human IgG γ chain using this Goat anti-Human IgG γ chain Antibody, Alkaline Phosphatase conjugate, Species Adsorbed validated for use in ELISA.

Research CategorySecondary & Control Antibodies

ELISA: 1:2,000-1:4,000.

Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

IgG is one of the most abundant protein in human serum. IgG is important for our defence against microorganisms and the molecules are produced by B-lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Immunogen

Epitope: IgG γ chain

Prepared from IgG γ chain. Pooled antisera from goats hyperimmunized with normal human IgG and human IgG paraproteins.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Affinity Chromatography

Purified by affinity chromatography on pooled human IgG covalently linked to agarose. Liquid in 50mM Tris/1mM MgCl₂/50% Glycerol, pH 8.0, containing 0.1% NaN₃ as preservative.

Specificity

The antibody reacts with the heavy chain of human IgG as demonstrated by ELISA and flow cytometry.

Reacts with with the heavy chain of human IgG. Absorbed for human IgM, IgD and IgA.

Storage and Stability

Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. For long term storage, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.