Antibody Adsorbant
Absorbed for mouse IgG1, IgG2a, IgG2b, IgG3 and IgA, pooled human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins.
Application
Research Sub CategorySecondary Antibodies Adsorbed for Dual LabelingFragment Specific Secondary Antibodies
Immunofluorescence: ≤0.1 µg/106 cells
ELISA
Optimal working dilutions must be determined by the end user.
Goat anti-Mouse IgM µ chain Antibody, Cy5 conjugate, Species Adsorbed detects level of Mouse IgM µ chain & has been published & validated for use in ELISA & IF.
Research CategorySecondary & Control Antibodies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
IgM constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as ′natural′ blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. The gene for the mu constant region contains four domains separated by short intervening sequences.
Immunogen
Prepared from mouse IgM µ chain. Pooled antisera from goats hyperimmunized with mouse IgM paraproteins.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Physical form
Purified by affinity chromatography on mouse IgM covalently linked to agarose. Liquid in 1.0 mL PBS/NaN3.
Affinity Chromatography
Specificity
The antibody reacts with the heavy chain of mouse IgM as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.
Storage and Stability
Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. Do not freeze.
This product has met the following criteria to qualify for the following awards: