Anti-Mouse IgM; Human ads;Gt;BIO 1MG

Application

ELISA: 1:5,000-1:20,000

Optimal working dilutions must be determined by the end user.

Research Sub CategorySecondary Antibodies Adsorbed for Dual LabelingFragment Specific Secondary Antibodies

This Goat anti-Mouse IgM µ chain Antibody, biotin conjugate, Species Adsorbed is validated for use in ELISA for the detection of Mouse IgM µ chain.

Research CategorySecondary & Control Antibodies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

IgM constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as ′natural′ blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. The gene for the mu constant region contains four domains separated by short intervening sequences.

Immunogen

Prepared from purified mouse IgM µ chain. Pooled antisera from goats hyperimmunized with mouse IgM paraproteins.

Epitope: IgM µ chain

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified by affinity chromatography on mouse IgM covalently linked to agarose. Liquid in 2.0 mL PBS/NaN₃.

Affinity Chromatography

Specificity

The antibody reacts with the heavy chain of mouse IgM as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.

Reacts with heavy chain of mouse IgM. Absorbed for mouse IgG1, IgG2a, IgG2b, IgG3 and IgA, pooled human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins. Reactivity with other species has not been determined.

Storage and Stability

Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt.