Application

This Donkey anti-Mouse IgG Antibody, Cy3 conjugate, Species Adsorbed is validated for use in IF for the detection of Donkey Mouse IgG.

Research CategorySecondary & Control Antibodies

Research Sub CategorySecondary Antibodies Adsorbed for Dual Labeling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: variable

Physical form

ImmunoAffinity Purified

Lyophilized. Buffer = 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 with 15 mg/mL BSA, and 0.05% sodium azide.

Specificity

Specific for mouse IgG, heavy and light chain.

Storage and Stability

Store lyophilized material at 2–8°C for up to 1 year from date of shipment. After reconstitution this product is stable for several weeks at 2–8°C as an undiluted liquid. DO NOT FREEZE. Store away from light. For extended storage after reconstitution, add an equal volume of glycerol (ACS grade or better) to make a final concentration of 50% glycerol and store at 2–8°C for up to 6 months. Avoid repeated warming/cooling cycles. Please note that the concentration of protein and buffer salts will decrease to one-half of the original after adding glycerol.