Application

1:50 - 1:200 for most applications.

Research CategorySecondary & Control Antibodies

Research Sub CategorySecondary Antibodies Adsorbed for Dual Labeling

This Donkey anti-Rat IgG Antibody, FITC conjugate, Species Adsorbed is validated for use in IF for the detection of Donkey Rat IgG.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Highly purified Rat IgG isolated from pooled normal Rat Serum.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Freeze-dried powder.

Specificity

Antibody was isolated from whole goat antiserum by antigen affinity chromatography. Monospecific by IEP against whole rat serum. Reacts with heavy chain of Rat IgG, as well as the light chains from most rat immunoglobulin classes.

To ensure minimal cross-reactivity to non-target immunoglobulin, the antibody was solid-phase absorbed against serum proteins from bovine, chicken, goat, guinea pig, hamster (Syrian), horse, human, rabbit, and sheep.

F/P RATIO:

8.9 µg/mg; 3.3 moles FITC per mole IgG

Storage and Stability

Store at +2-8°C. Do not freeze. After opening, restore to 0.4mL with distilled water. As an undiluted liquid the product is stable at 2-8°C for several weeks. For extended storage after reconstitution, we suggest the addition of an equal volume of glycerol to make a final glycerol concentration of 50% followed by storage at -20°C. Working aliquots should be discarded after use. Expiration Date: 1 year from reconstitution.