Analysis Note

Positive ControlIMR32 cells

Application

ELISA (see comments)Immunoblotting (1-5 g/ml)

General description

This Anti-TLR7 Mouse mAb (2C9) is validated for use in ELISA, Immunoblotting for the detection of TLR7.

Purified mouse monoclonal antibody. Recognizes the ~120 kDa TLR7 protein.

Recognizes the ~120 kDa TLR7 protein in IMR32 cells.

Immunogen

A recombinant polypeptide corresponding to amino acids of 27-127 human TLR7, expressed as a GST fusion protein

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Regulatory Review (Z)