Application

Research Sub CategoryAll

Western Blotting Analysis: Performance of a representative lot was validated at a 1:4,000 dilution for target band labeling following a mouse monoclonal GAPDH antibody (Cat. No. MAB374) in Western blotting application using 10 µg of HeLa cell lysate.

Research CategorySecondary & Control Antibodies

Sheep Anti-Mouse IgG, Species absorbed, HRP conjugate, Cat. No. AC111P, is a secondary antibody HRP conjugate, validated for use in Western Blotting application with little or no cross-reactivity toward mouse IgA & IgM, chicken IgY or IgG from human, rat, rabbit, guinea pig, and hamster species.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Immunoglobulin G (IgG) is part of the family of glycoprotein immunoglobulins which stage immune responses against pathogenic organisms. IgG contributes to the immune response by mobilizing innate immune effector cells and inducing and pro-inflammatory responses. Such proinflammatory responses are mediated by Fc receptors which interact with sialylated Fc fragments on IgG molecules.

Immunogen

Purified mouse IgG.

Legal Information

StabilZyme is a registered trademark of SurModics, Inc.

Linkage

Replaces: AP300P

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Ammonium Sulfate.

Purified sheep polyclonal antibody in PBS with StabilZyme^® HRP Conjugate Stabilizer and 0.05% Proclin.

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: Performance of this HRP-conjugated secondary antibody (Cat No. AC111P) is validated at a 1:4,000 dilution for target band labeling following a mouse monoclonal GAPDH antibody (Cat. No. MAB374) in Western blotting application using 10 µg of A431 cell lysate.

Specificity

This antibody has been species absorbed and tested by ELISA prior to conjugation to ensure minimal cross-reaction toward chicken IgY or IgG from human, rat, rabbit, guinea pig, and hamster species, but may cross-react with immunoglobulins from other species.

Based on ELISA analysis using the unconjugated antibody (Cat. No. AC111), this antibody reacts with whole molecule mouse IgG (IgG>>IgG2a>IgG>>>>IgG1) with minimal or no cross-reactivity toward mouse IgA or IgM.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~150.0 kDa observed.