Analysis Note

ControlRamos cell lysate

Application

Detect Ena/VASP-like protein using this Anti-Ena/VASP-like protein Antibody validated for use in WB & IC.

Research Sub CategoryCytoskeleton

Research CategoryCell Structure

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Ena/VASP-like protein in NIH/3T3 cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Ena (VASP-like protein) is involved in many different cellular functions including cell migration and axon guidance. Studies have shown that Ena expression is significantly increased in breast cancer and that the level of expression is associated with tumor stage. Ena has also been linked to inner ear and hair cell development.

Immunogen

Epitope: EVH2 region

KLH-conjugated linear peptide corresponding to the EVH2 region of human Ena/VASP-like protein.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in Ramos cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Ena/VASP-like protein in 10 µg of Ramos cell lysate.

Specificity

This antibody recognizes Ena/VASP-like protein at the EVH2 region.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~46 kDa observed.