Analysis Note

ControlHeLa cell lysate

Application

Research CategoryCell Structure

Detect Dynamin-2 using this Anti-Dynamin-2 Antibody validated for use in WB.

Research Sub CategoryCytoskeletal Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Dynamin-2 is a ubiquitously expressed, membrane associated protein belonging to the Dynamin protein family of GTPases, a group consisting of three isoforms with distinct tissue specificity and function. Dynamin localizes to plasma membrane-coated pits by its interactions with amphiphysin through the dynamin PRD domain. Dynamin-2 is a component in the manufacture of microtubule bundles, and is capable of both binding and hydrolyzing GTP. It is also likely to be involved in the trafficking mechanisms for vesicles, especially those pertaining to endocytosis. Defects in the expression of Dynamin-2 have been shown to cause Charcot-Marie-Tooth disease dominant intermediate type B (CMTDIB), and centronuclear myopathy autosomal dominant (ADCNM). There is also some interest in Dynamin-2 as a potential regulator for adenovirus targeting cancer therapies.

Immunogen

KLH-conjugated linear peptide corresponding to human Dynamin-2.

Physical form

Rabbit polyclonal serum containing 0.5% sodium azide.

Unpurified

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1:1,000 dilution of the antibody detected Dynamin-2 in 10 µg of HeLa cell lysate.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~ 98 kDa observed