Application

Immunocytochemistry Analysis: 5 µg/mL from a representative lot detected KIF5 in K562 cells.

Immunofluorescence Analysis: 20 µg/mL from a representative lot detected KIF5 in K562 cells.

Research Sub CategoryCytoskeleton

Research CategoryCell Structure

Anti-KIF5 Antibody is an antibody against KIF5 for use in western blotting, ICC & immunofluorescence.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Kinesin heavy chain isoform 5A, also known as Kinesin heavy chain neuron-specific 1 or Neuronal kinesin heavy chain (NKHC), and encoded by the gene name KIF5A or NKHC1, are members of the kinesin superfamily of proteins (KIFs). These proteins are microtubuledependent motors that slide along microtubules and transport cellular organelles and mRNA to different parts of the cell. In neurons, KIF5 performs this role in an anterograde fashion from the neuronal cell body to axonal or dendritic terminals. KIF5 was initially differentiated from the ubiquitously expressed kinesin as being highly enriched in subsets of neurons and selectively concentrated in the cell body. KIF5 has at least three known isoforms; KIF5A (117 kDa), KIF5B (39 kDa), and KIF5C (39 kDa). Mutations in one of the isoforms of KIF5 (KIF5A) is thought to be involved in hereditary spastic paraplegias

Immunogen

KLH-conjugated synthetic peptide corresponding to the C-terminus of human KIF5.

Epitope: C-terminus

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected KIF5 in K562 cell lysate.

Specificity

This antibody recognizes all isoforms of KIF5.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~116 kDa observed. Uncharacterized bands may be observed in some lysate(s).