Analysis Note

ControlHeLa cell lysate

Application

Immunocytochemistry Analysis: 5 ug/mL from a representative lot detected IL-1RAcP in HeLa cells.

Research Sub CategoryCytokines & Cytokine Receptors

Research CategoryCell Structure

Detect Interleukin Receptor using this rabbit polyclonal antibody, Anti-IL-1RAcP Antibody validated for use in western blotting & ICC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Interleukin-1 receptor accessory protein (IL-1RAcP), also known as Interleukin-1 receptor 3 (IL-1R-3, IL-1R3) forms a complex with IL-1RI in response to IL-1 treatment. The IL-1 receptor-associated kinase (IRAK), which mediates activation of NF-kB inducing kinae (NIK) and of NF-kB, recruites to the IL-1R complex through IL-1 RAcP. IL-1 activation of stress-activated protein kinase and of acid sphingomyelinase also requires IL-1RAcP. Like IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated cellular response. IL-1RAcP is expressed in many tissues.

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminus of human IL-1RAcP.

Epitope: C-terminus

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected IL-1RAcP in 15 µg of HeLa cell lysate.

Specificity

This antibody recognizes the C-terminus of IL-1RAcP.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~66 kDa observed. Uncharacterized bands may appear in some lysate(s).