Anti-Autotaxin

Analysis Note

ControlOligodendrocyte precursor cell lysate

Application

Detect Autotaxin using this Anti-Autotaxin Antibody validated for use in WB, IH(P).

Western Blot Analysis: 0.5 µg/mL from a representative lot detected Autotaxin in 10 µg of oligodendrocyte precursor cell lysate. Western Blot performed with Tween.

Immunohistochemistry Analysis: A 1:400 dilution from a representative lot detected Autotaxin in human cerebral cortex and ductal carcinoma tissues.

General description

Autotaxin (also known as Enpp2), an exo-enzyme that is upregulated in various cancers such as breast and lung cancer, is a secreted protein integral to the production of the lipid signaling molecule, lysophosphatidic acid (LPA). Wutotaxin is synthesized as a pre-pro-enzyme and stimulates cell proliferation, cell motility, and angiogenesis. Studies have shown increased expression of autotaxin in tumors is associated with increased tumor aggressiveness.

Immunogen

KLH-conjugated linear peptide corresponding to human Autotaxin at the substrate binding region.

Epitope: Substrate binding region

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Quality

Evaluated by Western Blot in oligodendrocyte precursor cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Autotaxin in 10 µg of oligodendrocyte precursor cell lysate. Western Blot performed without Tween.

Specificity

This antibody recognizes Autotaxin at the substrate binding region.

Target description

~100/125 kDa observed.
3 isoforms are produced by alternative splicing with molecular weights of 99, 105, and 101 kDa The calculated molecular weight of this protein is 99 kDa, but can be observed at ~125 kDa due to high glycosylation. An uncharacterized band may appear at ~35 kDa in some lysates.