Anti-Telo-Collagen Type I; A1/COL1A1

Application

Research CategoryCell Structure

Western Blotting Analysis: A representative lot detected Telo-Collagen Type I, A1/COL1A1 in mouse lung and skin tissue lysate.
Flow Cytometry Analysis: A representative lot detected Telo-Collagen Type I, A1/COL1A1 in mouse lung tissue.
Immunohistochemistry Analysis: A representative lot detected Telo-Collagen Type I, A1/COL1A1 in mouse lung tissue.

Anti-Telo-Collagen Type I Antibody, A1/COL1A1 is an antibody against Telo-Collagen Type I for use in Western Blotting, Flow Cytometry, Immunohistochemistry.

Research Sub CategoryAdhesion (CAMs)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Collagen alpha-1(I) chain (UniProt P02452; also known as Alpha-1 type I collagen) is encoded by the COL1A1 gene (Gene ID 1277) in human. Collagen is the major component of the extracellular matrix (ECM) and form the fibrils of tendons, ligaments, and bones. Type I collagen consists of two alpha I chains and one alpha 2 chain. Alpha-1 type I collagen is initially produced as an 1464-amino acid prepro-form with a signal peptide sequence (a.a. 1-22) and two propeptide sequences (a.a. 23-161 and a.a. 1219 –1464), the removal of which yields the mature alpha-1(I) chain. The mature alpha-1(I) chain is composed mostly of a large triple-helical region (a.a. 179-1192) sandwiched between two nonhelical segments known as the N-terminal telopeptide (a.a. 162-178; numbering based on the prepro-form) and the C-terminal telopeptide (a.a. 1193-1218; numbering based on the prepro-form). Collagen can be extracted from tissue via either enzymatic or non-enzymatic means. Collagen extracted using the proteolytic enzyme pepsin corresponds to the large triple-helical region, referred to as atelocollagen because both the N- and C-terminal telopeptides have been cleaved off by pepsin. On the other hand, collagen preparations obtained with non-enzymatic means (e.g. by acid extraction) have the intact telopeptides at both ends.

Immunogen

Epitope: C-terminal telopeptide region

Linear peptide corresponding to sequence derived from human Collagen Type I, A1/COL1A1 C-terminal telopeptide region.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in mouse liver tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Telo-Collagen Type I, A1/COL1A1 in 1 µg of mouse liver tissue lysate.

Specificity

Cat. No. ABT256 Anti-Telo-Collagen Type I, A1/COL1A1 was raised against a sequence derived from the C-terminal telopeptide (not the N-terminal telopeptide) region. This polyclonal antibody will detect prepro-, pro-, and mature collagen alpha-1(I) chain, but not atelocollagen prepared with proteolytic enzymes due to the absence of the C-terminal telopeptide sequence.

Predicted to react with a broad range of species based on high sequence homology, including mammalian, avian, and amphibian species.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~180 kDa observed