Anti-Actin; Alexa Fluor 488 Conjuga

Application

This rabbit polyclonal Anti-Actin, Alexa Fluor^® 488 Conjugate, Cat. No. ABT1485-AF488 is validated for use in Flow Cytometry, Immunocytochemistry and Immunofluorescence applications for the detection of Actin.

Research CategoryCell Structure

Flow Cytometry Analysis: 2 µL from a representative lot detected actin in one million HeLa cells.

Immunofluorescence Analysis: A 1:150 dilution from a representative lot detected actin in human heart and skin muscle tissue sections.

The unconjugated antibody (Cat. No. ABT1485) and Alexa Fluor^® 647-conjugated antibody (Cat. No. ABT1485-AF647) are also available for flow cytometry, immunocytochemistry, immunofluorescence, immunohistochemistry and Western blotting applications.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Actins are globular multi-functional proteins that serve as the basic building blocks of cytoskeletal microfilaments and are among the most conserved eukaryotic proteins. Six actin types exist, skeletal muscle alpha-actin (ACTA1), smooth muscle alpha-actin (ACTA2), cytoplasmic beta-actin (ACTB), cardiac muscle alpha-actin (ACTC1), cytoplasmic gamma-actin (ACTG1), and smooth muscle gamma-actin (ACTG2, a.k.a. ACTA3). Although actins show more than 90% overall sequence homology, isotypes do show spatial, temporal, and tissue-specific expresson patterns and only 50-60% homology is found in their 18 N-terminal residues. Cytoplasmic β and γ-actins are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types. Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins. The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt. In additon to serving as the basic building blocks of cytoskeletal microfilaments, actins are involved in multiple nuclear functions and adopt specific conformations that are important for their association with nuclear actin-binding proteins.

Immunogen

KLH-conjugated linear peptide corresponding to the N-terminal end sequence of mature skeletal muscle alpha-actin (ACTA1).

Epitope: N-terminus

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified.

Purified rabbit polyclonal antibody conjugate in PBS with 1.5% BSA and 0.05% sodium azide.

Quality

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected both cytoplasmic and nuclear actin immunoreactivity in HeLa cells.

Specificity

Broad species reactivity expected due to 100% sequence homology, including Bovine, Horse, Non-Human Primate, Porcine, Rabbit, and Rat.

This Alexa Fluor^® 488 conjugate was prepared from purified rabbit polyclonal antibody (Cat. No. ABT1485) raised against skeletal muscle alpha-actin (ACTA1) N-terminal end sequence that shares 80% sequence identity in the corresponding region with cardiac muscle alpha-actin (ACTC1), smooth muscle alpha-actin (ACTA2), and cytoplasmic gamma-actin (ACTG1). Immunogen sequence is not found in smooth muscle gamma-actin (ACTG2, a.k.a. ACTA3) and cytoplasmic beta-actin (ACTB).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~42 kDa observed.