Application

Anti-Neurocan, C-terminal, Cat. No. ABT1347, is a rabbit polyclonal antibody that detects Neurocan core protein and has been tested for use in Immunofluorescence, Immunohistochemistry, and Radioimmunoassay.

Research CategoryCell Structure

Radioimmunoassay Analysis: A representative lot detected Neurocan in rat brain (Milev, P., et. al. (1998). Biochem Biophys Res Commun. 247(2):207-12).

Immunohistochemistry Analysis: A representative lot detected Neurocan in E13 and E14 mouse ventral diencephalon and retina in C57 mouse embryos (Leung, K.M., et. al. (2004). Brain Res Dev Brain Res. 152(1):1-10).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Neurocan core protein (UniProt: P55067; also known as 245 kDa early postnatal core glycoprotein, Chondroitin sulfate proteoglycan 3) is encoded by the Ncan (also known as Cspg3) gene in rat. Neurocan is a secreted protein of the aggrecan/versican proteoglycan family that is found in early postnatal and adult brain. During early postnatal development neurocan accounts for at least 20% of the protein of the soluble brain proteoglycans. Neurocan mostly contains chondroitin sulfate, but also has N-linked and O-linked oligosaccharides. It is shown to be involved in the modulation of cell adhesion and migration and regulates neurite outgrowth. Neurocan interact with several cell surface molecules, such as N-CAM, L1/Ng-CAM, etc., and modulates the cell-binding and neurite outgrowth promoting activates of these molecules. Neurocan is synthesized with a 22 aa signal peptide that is subsequently cleaved to produce the mature form. Neurocan contains one C-type lectin domain, two EGF-like domains, one Ig-like V type domain, two link domains and one Sushi (CCP/SCR) domain. (Ref.: Rauch, U., et al. (2001) Cell Mol. Life Sci. 58(12-13):1842-56; Zhou, XH et al. (2001). Mol. Cell Biol. 21(17): 5970-78).

Immunogen

Epitope: C-terminus

GST-tagged recombinant fragment corresponding to 212 amino acids from the C-terminal half of rat Neurocan core protein.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Unpurified

Rabbit polyclonal antiserum with 0.02% sodium azide.

Unpurified

Quality

Evaluated by Immunofluorescence in E14 rat embryo, E14 rat neocortex, and adult rat brain hippocampus cells.

Immunofluorescence Analysis: A 1:500 dilution of this antibody detected Neurocan in E14 rat embryo, E14 rat neocortex, and adult rat brain hippocampus cells.

Specificity

This rabbit polyclonal antibody detects Neurocan in fetal and adult rat brain.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

135.55 kDa calculated.