Anti-FucT-III

Analysis Note

ControlHuman Liver Tissue Lysate

Application

Anti-FucT-III Antibody detects level of FucT-III & has been published & validated for use in Western Blotting.

Western Blot Analysis: 1 µg/mL from a representative lot detected FucT-III on 10 µg of transfected lysate and did not detect the non transfected lysate.

Research CategoryCell Structure

Research Sub CategoryGlycobiology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Alpha-(1,3)-fucosyltransferase 3 (FucT-III; Galactoside 3(4)-L-fucosyltransferase) is highly expressed in the small intestine, colon, stomach, and kidney. It is a member of the glycosyltransferase family of proteins which are involved in a glycosylation pathway that attaches fucose to Lewis epitopes of target molecules. This type of post-translational modification allows modified (fucosylated) molecules to recognize specific target substrates. Fucosyltransferases may therefore play an important role in communication at the cell surface. FucT-III may play a role in blood group determination as it is involved in the expression of antigens such as Lewis B, sialy Lewis X, Vim-2, and Lewis A.

Immunogen

KLH-conjugated linear peptide corresponding to the lumenal region of human FucT-III.

Epitope: Lumenal region

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in human liver tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected FucT-III on 10 µg of human liver tissue lysate.

Specificity

This antibody recognizes FucT-III at the lumenal region.

No homology to Rat and Mouse was found.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~50 kDa observed. The calculated molecular weight of this protein is 42 kDa, but can be observed at ~50 kDa due to high glycosylation. (Christensen, L, et al. (2000). Glycobiology. 10(9):931-939.)