Anti-CKAP5

Analysis Note

ControlA431 cells

Application

Immunocytochemistry Analysis: A 1:250 dilution from a previous lot detected CKAP5 in RPE1 cells.

Research Sub CategoryCytoskeleton

Research CategoryCell Structure

Anti-CKAP5 Antibody is an antibody against CKAP5 for use in IC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Cytoskeleton-associated protein 5 (CKAP5) is a member of the TOG/XMAP215 family containing heat repeats and plays a role in kinetochore mircotubule protection from depolymerization by MCAK. CKAP5 is the homolog of the Xenopus protein, XMAP25 (also known as chTog), which plays an essential role in centrosomal assembly. CKAP5 is localized in the perinuclear cytoplasm during interphase and at the spindle poles and centrosomes during mitosis. Studies show CKAP5 interacts with TACC1, TACC3, and tubulin leading to the hypothesis that it functions in spindle microtuble organization. Hepatomas and colonic tumors have shown overexpression of CKAP5.

Immunogen

KLH-conjugated linear peptide corresponding to human CKAP5.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected CKAP5 in A431 cells.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

226 kDa calculated.
Three isoforms are produced by alternative splicing with molecular weights of 225, 218, and 226 kDa