Application

Immunocytochemistry Analysis: A representative lot detected ACAT-2 in human liver tissue (Parini, P., et al. (2004). Circulation. 2004;110:2017-2023).
Western Blot Analysis: A representative lot detected ACAT-2 in human liver lysate (Parini, P., et al. (2004). Circulation. 2004;110:2017-2023)

Research CategorySignaling

This Anti-ACAT-2 Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of ACAT-2.

Research Sub CategoryLipid Metabolism & Weight Regulation

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Acyl-coenzyme A:cholesterol acyltransferase 2 (ACAT-2), also known as Sterol O-acyltransferase 2
(SOAT2), is a synthetic enzyme that is important in lipoprotein assembly, steroid metabolism, and dietary cholesterol metabolism and absorption. ACAT-2 is expressed primarily in the liver and intestine in enterocytes in their endoplasmic reticulum. ACAT-2 esterifies cholesterol allowing it to be more efficiently packaged and transported. Free cholesterol can be taken up by lipoproteins but it is confined to the outer surface of the lipoparticle. Esterification allows internal packaging and much more efficient transport through the blood stream. Gene expression of ACAT-2 is partially regulated by the transcriptional repressor TGIF1 as well as other factors such as thyroid hormone expression and microRNA miR181d.

Immunogen

Recombinant protein corresponding to Human ACAT-2.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit Polyclonal in buffer containing 20 mM NaPO4, 28 mM NaCl and 0.05% sodium azide.

Antigen Affinity Purified

Quality

Evaluated by Western Blotting in human lung tissue lysate using a non-reduced gel.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ACAT-2 in 10 µg of human lung tissue lysate using a non-reduced gel

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 55 kDa observed, uncharacterized bands may appear at lower molecular weight