Application

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected PRMT8 in human and mouse brain tissuse, and mouse thalamus.

This Anti-PRMT8 Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of PRMT8.

Research CategoryEpigenetics & Nuclear FunctionEpigenetics & Nuclear Function

Research Sub CategoryChromatin BiologyHistones

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Asymmetric dimethylation of arginine side chains in proteins is a frequent posttranslational modification, catalyzed by type I protein arginine methyltransferases (PRMTs). PRMT8 is a membrane-associated arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA). PRMT8 can form dimers with PRMT2 or PRMT1 and interacts with FYN kinase. PRMT8 is brain specific and it is a vertebrate restricted paralogue of PRMT1. In the past, arginine methylation was mainly observed on abundant proteins such as RNA-binding proteins and histones, but recent advances have revealed a plethora of arginine-methylated proteins implicated in a variety of cellular processes including signal transduction, epigenetic regulation and DNA repair pathways. PRMT8 has been shown to be critical for proper neuronal development and its expression is regionally restricted in the adult suggesting that PRMT8 is chiefly involved in the somatosensory and limbic systems, and a part of the motor nervous system development and maintenance.

Immunogen

KLH-conjugated linear peptide corresponding to the SAM-dependent methyltransferase PRMT-type domain of Human PRMT8.

Epitope: C-terminal region of SAM-dependent methyltransferase PRMT-type domain

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Antigen Affinity Purified

Quality

Evaluated by Western Blotting using human fetal brain tissue lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected PRMT8 in 10 µg of human fetal brain tissue lysate.

Specificity

Other homologies: Homology: predicted to react with rat.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~48 kDa observed