Application

Western Blotting Analysis: A representative lot detected methylglutaryl-lysine in Western Blotting applications (Wagner, G.R., et. al. (2017). Cell Metab. 25(4):823-837; Anderson, K.A., et. al. (2017). Cell Metab. 25(4):838-855.e15).

Research CategorySignaling

Anti-methylglutaryl-lysine, Cat. No. ABS2120, is a rabbit polyclonal antibody that detects 3-methylglutaryl-lysine conugated proteins and has been tested for use in Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Acetylation of lysine residues is proteins has emerged as an important mechanism in regulation and coordination of normal and pathophysiological cell metabolism. Lysine 3-methylglutaryl is a newer post-translational protein modification that occurs under normal basal conditions. In murine models of HMG-CoA lyase deficiency, higher levels of protein lysine 3-MGylation are observed. This rabbit polyclonal antibody specifically detects chemically modified proteins containing 3-methylglutaryl lysine modifications, but not unmodified proteins. (Ref.: Wagner, GR et al. (2017). Cell Metabolism 25(4); 823-837).

Immunogen

3-methylglutaryl (MG)-lysine conjugated to BSA.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in PBS with 0.1% sodium azide.

Quality

Evaluated by Western Blotting with methyl glutaryl BSA conjugate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected 3-methylglutaryl (MG)-lysine in methyl glutaryl BSA conjugate.

Specificity

This rabbit polyclonal antibody detects 3-methylglutaryl (MG)-lysine conjugated to BSA.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~75 kDa observed (BSA conjugate). Uncharacterized bands may be observed in some lysate(s).