Analysis Note

ControlMouse brain tissue lysate

Application

Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected DARC in mouse brain tissue.

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Neuroscience

Detect Duffy antigen/chemokine receptor using this rabbit polyclonal antibody, Anti-DARC Antibody validated for use in western blotting & IHC (Paraffin).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Duffy antigen/chemokine receptor (DARC), also known as Fy glycoprotein (GpFy), is a multi-pass membrane protein belonging to the G-protein coupled receptor Duffy family. DARC binds, with high affinity, several chemokines without transducing any signal, suggesting it may modulate the signals normally induced by these chemokines. DARC is found in adult kidney, adult spleen, bone marrow, and fetal liver. It is expressed along postcapillary venules throughout the body, except in the adult liver.

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminus of human DARC.

Epitope: C-terminus

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected DARC in 15 µg of mouse brain tissue lysate.

Specificity

This antibody recognizes the C-terminus of DARC.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~23 and 35 kDa observed. Uniprot describes two isoforms at ~35 and ~36 kDa