Anti-PDE11A

Analysis Note

ControlHuman hippocampus tissue lysate

Application

Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected PDE11A in normal mouse midbrain tissue.

Research CategoryNeuroscience

Research Sub CategoryGPCR, cAMP/cGMP & Calcium Signaling

Detect PDE11A using this Anti-PDE11A Antibody validated for use in WB, IH.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The PDE11A gene encodes for a dual-specificity Phosphodiesterase (PDE), which is expressed in the adrenal cortex and is partially inhibited by tadalafil and other PDE inhibitors. PDEs are known to catalyze cAMP and cGMP to 5'-AMP and 5'GMP, respectively and thereby regulate the intracellular concentration of these nucleotides. Loss-of-function mutation of the PDE11A gene is shown to increase cAMP signaling and cAMP-responsive element binding protein (CREB) phosphorylation, two conditions which are associated with adrenocortical hyperplasia, adenomas, and Cushing syndrome.

Immunogen

KLH-conjugated linear peptide corresponding to the GAF domain of human PDE11A.

Epitope: GAF domain.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in human hippocampus tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected PDE11A in 10 µg of human hippocampus tissue lysate.

Specificity

This antibody recognizes GAF domain of PDE11A.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~104 kDa observed. Uniprot states four isoforms at a molecular weight of ~105 kDa, ~78 kDa, ~66 kDa, and ~56 kDa This antibody can only recognize the ~105 kDa and the ~78 kDa isoforms.