Analysis Note

ControlMouse brain tissue lysate

Application

Research CategoryNeuroscience

Western Blot Analysis: 2 µg/mL from a representative lot detected LGI1 in 10 µg/mL of HEK293 cell lysate.

Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected LGI1 in mouse cerebellum and rat cortex tissues.

Research Sub CategoryDevelopmental Neuroscience

Detect the LGI1 protein using this Anti-LGI1 validated for use in WB & IHC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The leucine-rich, glioma inactivated gene 1 (LGI1) was first identified as tumor suppressor gene for glioma and may play a role in other cancers. LGI1 is a member of a family of highly related proteins containing leucine-rich repeats (LRRs) which are very similar to other transmembrane signaling molecules and receptors. LGI1 serves as a ligand to ADAM22, a metalloprotease localized at the synapse. Mutations in LGI1 account for nearly half of autodominant lateral temporal epilepsy (ADTLE), an epileptic syndrome characterized by focal seizures with predominant auditory symptoms.

Immunogen

KLH-conjugated linear peptide corresponding to human LGI1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rabbit polyclonal in buffer containing PBS with 0.02% sodium azide.

Format: Purified

Antigen Affinity Purified

Quality

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: 2 µg/mL of this antibody detected LGI1 in 10 µg/mL of mouse brain tissue lysate.

Specificity

This antibody recognizes only Isoform 1.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~64 kDa observed. Uniprot describes 3 isoforms at ~33 kDa, ~58 kDa, and ~64 kDa An uncharacterized band may appear at ~45 kDa in some lysates.