Anti-MANF

Analysis Note

ControlPC12 cell lysate

Application

Detect the MANF protein using this Anti-MANF validated for use in WB & IHC.

Research Sub CategoryNeurodegenerative Diseases

Research CategoryNeuroscience

Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected MANF in human midbrain and rat brain tissues.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

MANF, also known as ARMET, was initially identified as a protein containing an arginine-rich region that was highly mutated in a variety of tumors. More recently it was identified as a mesencephalic astrocyte-derived neurotrophic factor with selectivity for dopaminergic neurons, similar to glial cell line-derived neurotrophic factor (GDNF) and CDNF. In rat brain slices, MANF enhanced nigral gamma-aminobutyric acid release. Like GDNF and CDNF, MANF has selective neuroprotective activity for dopaminergic neurons suggesting that it may be indicated for the treatment of Parkinson's disease. Expression of MANF has also been shown to be induced during ER stress, suggesting that it may play a role in protein quality control during ER stress.

Immunogen

KLH-conjugated linear peptide corresponding to human MANF.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rabbit polyclonal in buffer containing PBS with 0.02% sodium azide.

Antigen Affinity Purified

Quality

Evaluated by Western Blot in PC12 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected MANF in 15 µg/mL of PC12 cell lysate.

Specificity

This antibody does not cross-react with CDNF.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~18 kDa observed. The calculated molecular weight is 21 kDa, however MANF has been shown as an ~18 kDa band in western blots. (Mizobuchi, N., et al. (2007). Cell Structure and Function. 32:41-50.). Uncharacterized bands may appear at ~25 kDa and ~100 kDa in some lysates.