Analysis Note
ControlHuman placenta tissue lysate
Application
Research CategoryNeuroscience
Anti-Aromatase Antibody is an antibody against Aromatase for use in WB.
Research Sub CategoryNeurochemistry & Neurotrophins
Western Blot Analysis: 1 µg/mL from a representative lot detected Aromatase in 10 µg of human testis.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Human Aromatase is a membrane-bound enzyme that is localized on the endoplasmic reticulum. While it is found in gonads and adrenal glands where it converts androgens into estrogens in a classical endocrine manner, it is also expressed in various tissues where it regulates local estrogen levels or estrogen/androgen ratios in an intracrine manner. Human Aromatase is known to have two major forms, characterized by one amino acid difference at amino acid position 264 [arginine (264R) and cysteine (264C)]. Abnormal Aromatase hyperactivity is a known cause for Aromatase excess syndrome (AES), which is characterized by excess levels of estrogen, heterosexual precocity in adolescent males and isosexual precocity in adolescent females. On the other hand, Aromatase deficiency (AROD) in Aromatase-deficient (ArKO) mice leads to increased adiposity, an inability to synthesize estrogen, birth defects, and genital-related developmental disorders. It is also reported to play a role in a complex immune-neuro-endocrine network management system in murine cysticercosis.
Immunogen
KLH-conjugated linear peptide corresponding to human Aromatase.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality
Evaluated by Western Blot in human placenta tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Aromatase in 10 µg of human placenta tissue lysate. Tween was not used in the Western Blot.
Specificity
Other homolgoies: Rat and Mouse (94% sequence homology).
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Target description
~58 kDa observed. UniProtKB/Swiss-Prot P11511 (CP19A_HUMAN) states a molecular weight of ~58 kDa
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