Analysis Note

ControlHuman cerebellum tissue lysate

Application

Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected NSG1 in rat cerebellum, human pons/midbrain, and rat hippocampus tissue.

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Signaling

Detect the NSG1 protein using this Anti-NSG1 validated for use in WB & IHC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Neuron-specific protein family member 1 (NSG1), is also known as Brain neuron cytoplasmic protein 1. It is responsible for dopamine receptor binding in the dopamine signaling pathway.

Immunogen

Epitope: Cytoplasmic domain

KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human NSG1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in human cerebellum tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected NSG1 in 10 µg of human cerebellum tissue lysate.

Specificity

This antibody recognizes the cytoplasmic domain of NSG1.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~20/17 kDa observed. An uncharacterized band may appear at ~12 kDa in some lysates. The calculated molecular weight is 21 kDa, however NSG1 has been shown as a ~20/17 kDa band in western blots (Catsicas, S., et al. (2004). Lausanne, EPFL. 1-162).