Analysis Note

ControlRat testis tissue lysate

Application

Anti-Dopamine D(1A) Receptor Antibody detects level of Dopamine D(1A) Receptor & has been published & validated for use in IH, WB, ELISA.

Research CategoryNeuroscience

Research Sub CategoryNeurotransmitters & Receptors

Immunohistochemistry Analysis: A 1:300 dilution from a previous lot detected D(1A) dopamine receptor in rat brain tissue.

ELISA Analysis: Tested in ELISA against antigen-coated plate. A 1:100 dilution from a previous lot detected dopamine D(1A) receptor peptide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Dopaminergic signals are thought to modulate corticostriatal synapses onto medium spiny projection neurons, and this plasticity is, in turn, thought to alter patterns of activity during learning. Subversion of these processes in clinical and rodent models is hypothesized to contribute importantly to addiction to psychostimulants, seizures, and movement disorders in Huntington's (HD) and Parkinson's disease (PD). Dopamine receptor D1, also known as DRD1, is the most abundant dopamine receptor (of the five types D1-D5) in the central nervous system and is critically involved in these processes and the modulation of D2 receptor mediated events. Coupled with G-protein, the D1a receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases including the key molecular switch, DARPP-32.

Immunogen

KLH-conjugated linear peptide of rat dopamine D(1A) receptor at and around the cytoplasmic domain.

Epitope: Cytoplasmic Domain

Linkage

Replaces: AB1765P;

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Quality

Evaluated by Western Blot in rat testis tissue lysate.

Western Blot Analysis: 2 µg/ml of this antibody detected dopamine D(1A) receptor in 10 µg of rat testis tissue lysate.

Specificity

This antibody recognizes D(1A) dopamine receptor.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 55 kDa is observed