ANTI-MECP2

Application

Anti-MeCP2 Antibody, Cat. No. ABN1728, is a highly specific rabbit polyclonal antibody that targets MeCP2 and has been tested in Immunofluorescence and Western Blotting.

Immunofluorescence Analysis: A 1:1000 dilution from a representative lot detected MeCp2 in mouse brain tissue sections (Courtesy of EnCor Biotechnology Inc.).

General description

Methyl-CpG-binding protein 2 (UniProt P51608; also known as MeCp-2 protein, MeCp2) is encoded by the MECP2 (also known as AS, MRXS13, MRXSL, PPMX, RTT, AUTSX3) gene (Gene ID 4204) in human. Methyl-CpG-binding proteins that are involved in gene expression regulation by targeting methylated DNA residues in gene promoters via a conserved methyl-CpG-binding domain (MBD; a.a. 90-162 of human MeCp2). Upon binding methylated CpG sites on the DNA strands, transcriptional repression domain (TRD; a.a. 207-492) then mediates association with SIN3A to recruit histone deacetylases (HDAC). The TRD region also contains a nuclear localization signal (NLS) sequence (a.a. 255-271) and a nuclear receptor co-repressor 2/NCoR2-interacting site (a.a. 269-309). MeCp2 protein is found in all cells in the body, including the brain, where it is found in high concentrations in neurons and is associated with maturation of the central nervous system (CNS) and in forming synaptic contacts. In contrast to other MBD family members, MECP2 is X-linked and subject to X chromosome inactivation. MECP2 gene mutations are linked to Rett syndrome (RTT), a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.

Immunogen

Linear peptide corresponding to human MeCp2 C-terminal end sequence.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody in buffer containing PBS with 0.03% sodium azide

Quality

Evaluated by Western Blotting in mouse brain nuclear extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected MeCp2 in 10 µg of mouse brain nuclear extract.

Specificity

This polyclonal antibody targets MeCp2 C-terminal end sequence conserved between spliced isoform A (isoform Beta) and isoform B (isoform Alpha).

Target description

~70 kDa observed. 52.44/52.31 kDa (human/mouse isoform A or Beta) and 53.32/53.58 kDa (human/mouse isoform B or Alpha) calculated. The larger-than-predicted band size is consistent with that reported in the literature (Jarrar, M.H., et al. (2003). J. Child Neurol. 18(10):675-682). Uncharacterized bands may be observed in some lysate(s).