Analysis Note
ControlRat basophilic leukemia (RBL) cell lysate
Application
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected NLRP6 (NALP6) in epithelial tissue of proximal and distal tubules of human kidney, and in human bone marrow tissue.
Anti-NLRP6 (NALP6) Antibody detects level of NLRP6 (NALP6) & has been published & validated for use in WB, IH(P).
General description
NRLP6 (NALP6) (also known as PYRIN-containing Apaf-1-like protein 5 or PYPAF5) belongs to a family of proteins that play a critical role in apoptosis and in inflammatory signaling. Its expression is highly restricted to granulocytes and T-cells. Co-expression of NALP6 with ASC results in synergistic activation of caspase-1-dependent cytokine processing, subsequent NF-kB activation, and downstream recruitment of PYPAF5 to punctate structures in the cytoplasm. Overlapping genes in NALP6/PYPAF5 locus encode two V2-type vasopressin isoreceptors: angiotensin-vasopressin receptor (AVR) and non-AVR.
Immunogen
Linear peptide corresponding to NACHT domain of human NLRP6 (NALP6).
Epitope: NACHT domain
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Quality
Evaluated by Western Blot in rat basophilic leukemia (RBL) cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected NLRP6 (NALP6) in 10 µg of rat basophilic leukemia (RBL) cell lysate.
Specificity
This antibody recognizes the NACHT domain of NLRP6 (NALP6).
Other homologies: Mouse (93% sequence homology).
Target description
~99 kDa observed
This product has met the following criteria to qualify for the following awards: