Analysis Note

ControlTHP-1 cell lysate

Application

Research Sub CategoryImmunological Signaling

Immunohistochemistry Analysis: A 1:4,000 dilution from a representative lot detected NLRP3 in human leukemia bone marrow and human tonsil tissue lysate.

Detect NLRP3 using this rabbit polyclonal antibody, Anti-NLRP3 Antibody validated for use in western blotting & IHC (Paraffin).

Research CategoryInflammation & Immunology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

NLRPs (Nucleotide-binding oligomerization domain, Leucine rich Repeat and Pyrin domain containing Proteins) are members of NLR (Nod-like receptors) protein family. NRLP genes are known to play important roles in both the mammalian reproductive system and the innate immune system. NLRP3 may function as an inducer of apoptosis, activator of NF-kappa-B signaling and activates caspase-1 in response to triggers such as bacterial or viral infection. NLRP3 defects are linked to familial cold autoinflammatory syndrome type 1 (FCAS1), Muckle-Wells syndrome (MWS) and chronic infantile neurologic cutaneous and articular syndrome (CINCA). NLRP3 is highly expressed in blood leukocytes, polymorphonuclear cells, osteoblasts and chondrocytes.

Immunogen

Epitope: DAPIN domain

KLH-conjugated linear peptide corresponding to the DAPIN domain of human NLRP3.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in THP-1 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected NLRP3 in 10 µg of THP-1 cell lysate.

Specificity

Other homologies: Porcine (80% sequence homology).

This antibody recognizes the DAPIN domain of NLRP3.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~112 kDa and ~75 kDa observed. Five isoforms are known to exist at 118 kDa, 112 kDa, 112 kDa, 106 kDa, and 84 kDa This protein has been observed at ~75 kDa in some cell lysates (Kummer, J. A., et al. (2007). J Histochem Cytochem. 55(5):443-452.). Uncharacterized bands may be observed in some cell lysates.