Anti-FcRn (FCGRT)

Application

Research CategoryInflammation & Immunology

Immunofluorescence Analysis: A representative lot detected FcRn (FCGRT) in mouse uterus and vagina (Li, Z., et. al. (2011). Proc Natl Acad Sci USA. 108(11):4388-93).

Western Blotting Analysis: A representative lot detected FcRn (FCGRT) in mouse liver, mouse uterus, and vagina tissue lysate (Li, Z., et. al. (2011). Proc Natl Acad Sci USA. 108(11):4388-93).

This rabbit polyclonal Anti-FcRn (FCGRT) antibody, Cat. No. ABF191, detects IgG receptor FcRn large subunit p51. It has been tested for use in Immunofluorescence and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

IgG receptor FcRn large subunit p51 (UniProt: Q61559; also known as FcRn, IgG Fc fragment receptor transporter alpha chain, Neonatal Fc receptor) is encoded by the Fcgrt (also known as Fcrn) gene (Gene ID: 14132) in murine species. FcRn is a single-pass type I membrane protein that binds to the Fc region of monomeric immunoglobulins gamma. FcRn complex consist of two subunits: p51 and p14 and contains one Ig-like C1-type (immunoglobulin-like) domain of 88 amino acids. FcRn mediates the selective uptake of IgG from milk and helps newborn animals to acquire passive immunity. In rodents, FcRn functions effectively in the neonatal period when it transports maternally derived IgG in ingested milk across the epithelial-cell layer of the proximal small intestine. However, in humans it transports maternal antibody to the fetus across the placenta. In rodent FcRn-IgG complexes are transcytosed across the intestinal epithelium and IgG is released from FcRn into blood or tissue fluids. FcRn also protects IgG from degradation providing long half-life of this class of antibody in the serum. (Ref.: Roopenian,, DC and Shreeram,, A (2007). Nat. Rev. Immunol. 7(9); 715-725).

Immunogen

GST-tagged recombinant fragment corresponding to 44 amino acids from the C-terminal region of murine IgG receptor FcRn large subunit p51.

Epitope: C-terminus

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

Format: Purified

Quality

Evaluated by Western Blotting in mouse liver tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected FcRn (FCGRT) in 10 µg of mouse liver tissue lysate.

Specificity

This polyclonal antibody detects murine IgG receptor FcRn. It targets an epitope within 44 amino acids from the C-terminal region.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~53 kDa observed; 40.09 kDa calculated. Uncharacterized bands may be observed in some lysate(s).