Application

Research Sub CategoryImmunoglobulins & Immunology

This Anti-Sharpin antibody is validated for use in WB, IP for the detection of Sharpin.

Research CategoryInflammation & Immunology

Western Blotting Analysis: A representative lot detected Sharpin in Splenic B cells were treated with an anti-CD40 antibody, anti-NEMO immunoprecipitates (IP) from TNF-a-treated MEFs HEK293T, Jurkat, cpdm MEFs, Cpdm MEFs transfected with control or HOIL-1L-specific siRNA, Brain lysates of Wt and cpdm mice, transfected cpdm MEFs, Lysates from Wt mice organsand splenic B and T cells from Wt and cpdm mice (Tokunaga, F., et al. (2010). Nature. 471:633-636).
Western Blotting Analysis: A representative lot detected Sharpin in HeLa cell lysate (Li, S., et al. (2013). Nature. 242-246).
Western Blotting Analysis: A representative lot detected Sharpin in HOIP-null MEFs, HOIP WT MEFs or HOIP N84A/Y93A MEFs (Takiuchi, T., et al. (2014). Genes to Cells. 1-19).
Western Blotting Analysis: A representative lot detected Sharpin in MEFs, BMDMs, and primary keratinocytes
from WT mice (Tamiya, H., et al. (2014). J. Immunology. 192:000-000).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Sharpin in HEK293T (Tokunaga, F., et al. (2010). Nature. 471:633-636).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Sharpin, also known as Sharpin Shank-associated RH domain-interacting protein, Shank-interacting protein-like 1, hSIPL1, and encoded by the gene name SHARPIN/ SIPL1/ PSEC0216, is component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. Sharpin is highly expressed in skeletal muscle and placenta and at lower levels in brain, heart, colon without mucosa, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes. Sharpin is up-regulated in various tumor tissues such as kidney, liver, ovary and pancreas tumors

Immunogen

GST-tagged recombinant protein corresponding to the N-terminus of human Sharpin.

Epitope: N-terminus

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein A purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Sharpin in 10 µg of HepG2 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~40 kDa observed. Anti-SHARPIN antibody demonstrated an approximately 40 kDa protein, the band is consistent with the predicted 39.9 kDa protein sequence for mouse. Uncharacterized band(s) may appear in some lysates.