Application

Anti-PGC-1 alpha, Cat. No. ABE868, is a highly specific rabbit polyclonal antibody that targets Peroxisome proliferator-activated receptor gamma coactivator 1-alpha and has been tested for use in Immunocytochemistry and Western Blotting.

Research CategoryEpigenetics & Nuclear Function

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected PGC-1 alpha in A431, HUVEC, and NIH/3T3 cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (UniProt: O70343; also known as PGC-1-alpha, PPAR-gamma coactivator 1-alpha, PPARGC-1-alpha) is encoded by the Ppargc1a (also known as Pgc1, Pgc1a, Ppargc1) gene (Gene ID: 19017) in murine species. PGC-1 alpha is a member of a family of transcription coactivators that plays a central role in the regulation of cellular energy metabolism. It is a homooligomeric transcriptional coactivator for steroid receptors and nuclear receptors that greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. It is expressed in muscles, liver, kidney, brown adipose tissue, heart, and brain. It can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis and is also reported to play an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. PGC-1 alpha is known to induce the expression of PPARGC1- and ESRR-induced regulator, muscle 1 (PERM1) in the skeletal muscle in an estrogen-related receptors, -alpha (ESRRA)-dependent manner. PGC-1 alpha is significantly induced in brown adipose tissue and skeletal muscle by exposure to cold and is also shown to be up-regulated in brown adipose tissue of obese leptin-deficient (ob/ob) and leptin-unresponsive (db/db) mice. PGC-1 alpha is also required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the co-activation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK.

Immunogen

KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminus region of murine PPAR-gamma coactivator 1-alpha.

Epitope: C-terminus

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

Quality

Evaluated by Western Blotting in human brown adipose tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected PGC-1 alpha in 10 µg of human brown adipose tissue lysate.

Specificity

This rabbit polyclonal antibody detects PGC-1 alpha in human and murine adipose tissue. it targets an epitope within 21 amino acids from the C-terminus region.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~100 kDa observed; 90.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).