Application

Research Sub CategoryNuclear Receptors

Western Blotting Analysis: 15 µg/mL from a representative lot detected YTHDF2 in WT HeLa cell lysate and YTHDF2 overexpressing lysate (Chuan He, Univerity of Chicago).
Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected YTHDF2 in HeLa cells (Chuan He, Univerity of Chicago).

This Anti-YTHDF2 antibody is validated for use in WB, IC for the detection of YTHDF2.

Research CategoryEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

YTH domain-containing family protein 2 (YTHDF2), also known as CLL-associated antigen KW-14, High-glucose-regulated protein 8, Renal carcinoma antigen NY-REN-2, is also encoded by the gene name YTHDF2 and HGRG8. YTHDF2 recognizes specifically and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs. Recent studies have shown that YTHDF2 may play an important role in the efficiency of mRNA splicing, processing and stability. Additionally, YTHDF2 has been shown to act as a regulator of mRNA stability. And in the process of binding to m6A-containing mRNAs, YTHDF2 has been implicated to localize to mRNA decay sites, such as processing bodies (P-bodies), which leads to mRNA degradation.

Immunogen

KLH-conjugated linear peptide corresponding to the localization of mRNA processing bodies (P-bodies) to human YTHDF2.

Epitope: Localization to mRNA processing bodies (P-bodies)

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in C6 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected YTHDF2 in 10 µg of C6 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~62 kDa observed. Uncharacterized band(s) may appear in some lysates.