Analysis Note

ControlA431 cell lysate

Application

Detect NO66 using this rabbit polyclonal antibody, Anti-Histone lysine demethylase NO66 Antibody validated for use in western blotting.

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryChromatin Biology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

NO66 is a lysine-specific demethylase that removes methyl groups from histone H3 at lysine 4 and lysine 36. This enzyme has been shown to more readily demethylate trimethylated H3 ′Lys-4′ (H3K4me3) and monomethylated H3 Lys4 (H3K4me1) consistent with its role in modulating the histone code. This protein is a highly conserved, constitutive nucleolar component that shows a dual localization pattern with strong enrichment in the granular part of nucleoli and in distinct nucleoplasmic entities.

Immunogen

KLH-conjugated linear peptide corresponding to human Histone lysine demethylase NO66 near the N-terminus.

Epitope: Near N-terminus

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Antigen Affinity Purified

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Histone lysine demethylase NO66 in 10 µg of A431 cell lysate.

Specificity

This antibody recognizes Histone lysine demethylase NO66 near the N-terminus.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~66 kDa observed