Analysis Note

ControlPMA(TPA) treated HeLa cell lysate

Application

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected c-Fos in rat pons and rat cerebellum tissues.

Immunofluorescence Analysis: A representative lot detected c-Fos in rat pons and rat cerebellum tissues.

Anti-c-Fos Antibody is a Rabbit Polyclonal Antibody for detection of c-Fos also known as Proto-oncogene c-Fos, Cellular oncogene fos & has been validated in WB & IHC.

General description

c-Fos is a member of a family of immediate early gene (IEG) transcription factors (other members include Jun and Egr-1) also identified as proto-oncogenes. The basal expression of c-Fos and other IEGs is typically low but increases relatively quickly and often dramatically (15-30 min for mRNA; 30-60 min for protein) in response to changes in cellular activity typically caused by external stimuli, such as stress or neuronal activation. Thus, these IEGs are being used to indicate specific activity driven cellular activation including the identification of neurons activated by various growth factors, neuroactive drugs, and correlated changes in behavioral or physiological states.

Immunogen

KLH-conjugated linear peptide corresponding to the N-terminus of human c-Fos.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Quality

Evaluated by Western Blot in PMA(TPA) treated HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected c-Fos in 10 µg of PMA(TPA) treated HeLa cell lysate.

Specificity

This antibody recognizes the N-terminus of c-Fos.

Target description

~60/56 kDa observed. This protein has multiple isoforms produced by alternative splicing ranging between 53 and 68 kDa (Jurado, J., et al. (2007). BMC Molecular Biology. 8:83-96.)